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A vector carrying the GFP gene (Green fluorescent protein) as a yeast marker for fermentation processes Scientia Agricola
Gomes,Luiz Humberto; Duarte,Keila Maria Roncato; Andrino,Felipe Gabriel; Giacomelli,Ana Maria Brancalion; Tavares,Flavio Cesar Almeida.
Contaminant yeasts spoil pure culture fermentations and cause great losses in quality and product yields. They can be detected by a variety of methods although none being so efficient for early detection of contaminant yeast cells that appear at low frequency. Pure cultures bearing genetic markers can ease the direct identification of cells and colonies among contaminants. Fast and easy detection are desired and morphological markers would even help the direct visualization of marked pure cultures among contaminants. The GFP gene for green fluorescent protein of Aquorea victoria, proved to be a very efficient marker to visualize transformed cells in mixed populations and tissues. To test this marker in the study of contaminated yeast fermentations, the GFP...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; GFP (green fluorescent protein); Fermentative processes.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000400018
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